Δ12 fatty acid desaturase gene from Geotrichum candidum in cheese: molecular cloning and functional characterization
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چکیده
منابع مشابه
A novel Δ12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115*
The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Δ12 and Δ15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Δ12-fatty acid desaturase gene from th...
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Numerous Δ12-, Δ15- and multifunctional membrane fatty acid desaturases (FADs) have been identified in fungi, revealing great variability in the enzymatic specificities of FADs involved in biosynthesis of polyunsaturated fatty acids (PUFAs). Here, we report gene isolation and characterization of novel Δ12/Δ15- and Δ15-FADs named CpFad2 and CpFad3, respectively, from the opportunistic pathogenic...
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Mycelium-bound lipase (MBL) was prepared using a strain of Geotrichum candidum isolated from local soil. At the time of maximum lipase activity (54 h), the mycelia to which the lipase was bound were harvested by filtration and centrifugation. Dry MBL was prepared by lyophilizing the mycelia obtained. The yield of MBL was 3.66 g/l with a protein content of 44.11 mg/g. The lipase activity and spe...
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The pathogenicity of the fungus Geotrichum candidum in humans has not been clearly defined. A patient with acute leukemia who developed a fatal disseminated G. candidum infection while neutropenic is described. At autopsy examination, this patient was misdiagnosed on the basis of histopathology as having disseminated candidiasis until G. candidum was isolated from postmortem culture specimens. ...
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Enzymes of essential amino acid biosynthesis pathways are the targets of choice for drug discovery as these pathways are absent in mammals. Inhibitors of these enzymes will have reduced toxicity problems for the vertebrate host organisms. The histidine biosynthesis pathway is an important biochemical pathway found in bacteria, archebacteria, fungi and plants but absent in human. In recent times...
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ژورنال
عنوان ژورنال: FEBS Open Bio
سال: 2018
ISSN: 2211-5463,2211-5463
DOI: 10.1002/2211-5463.12553